Regulation of schistosome egg granuloma formation: host-soluble L-selectin enters tissue-trapped eggs and binds to carbohydrate antigens on surface membranes of miracidia

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A method for the isolation of schistosome eggs and miracidia free of contaminating host tissues.

A novel method for the isolation of schistosome eggs and miracidia from livers of mice infected with Schistosoma japonicum or S. mansoni is described. The method employed collagenase B to degrade the interstitial matrix of mouse liver tissue, after which the schistosome eggs were separated from the liver cells by 2 single-step density centrifugations through Percoll. Using this procedure suffic...

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A Schistosoma mansoni cercarial antigen preparation (cercarial transformation fluid--SmCTF) was evaluated for detection of anti-schistosome antibodies in human sera in 4 collaborating laboratories. The performance of SmCTF was compared with that of S. mansoni egg antigens (SmSEA) in an indirect enzyme-immunoassay (ELISA) antigen assay, the latter being used routinely in 3 of the 4 participating...

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The study was conducted to determine the effects of turkey (Meleagris gallopavo)egg weight on certain egg traits and hatching performance. A total of 178 turkey eggs comprising of three different egg weight groups below 60 g (G I), 61 to 69 g (G II) and above 70 g (G III) were used for this study. Average egg weight (g), egg length (cm), egg breadth (cm) and egg volume (cm3) values increased wi...

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Elevated serum L-selectin levels and abnormal regulation of L-selectin expression on leukocytes in atopic dermatitis: soluble L-selectin levels indicate disease severity.

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ژورنال

عنوان ژورنال: Infection and Immunity

سال: 1996

ISSN: 0019-9567,1098-5522

DOI: 10.1128/iai.64.11.4700-4705.1996